Code: FOA	Time Slot/Poster Number: 08:30 - 9:00 am	Session: Raman Imaging III
Nonlinear vibrational imaging of tissues
Eric Potma
UC Irvine, Irvine, CA
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Summary
Nonlinear vibrational imaging, combined with other nonlinear imaging modalities such as second harmonic generation and two-photon excited fluorescence microscopy, constitutes a noninvasive visualization tool for examining unstained tissues. We will discuss several new developments and applications of nonlinear vibrational microscopy in the field of biomedical imaging.

Code: FOA	Time Slot/Poster Number: 09:00 - 9:30 am	Session: Raman Imaging III
Raman Imaging of Differentiating Cells
Cees Otto; Vishnu VardhanPully; Aufried Lenferink
University of Twente, Enschede, Netherlands
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Summary
Spontaneous Raman microspectroscopy is a powerful analytical method to detect chemical compounds inside living biological cells. The spatial resolution is determined by the wavelength of the exciting and emitted light. We have standardized our home-built platform around the 647.1 nm emission line from a Krypton-ion laser. A lateral resolution of 0.39 micrometer diameter has been achieved and voxel-sizes of just over 100 attoliter have been reached. The ability of Raman microspectroscopy to measure multiple chemical species simultaneously offers ample opportunity for correlative chemical imaging. This study of stem cell differentiation exemplifies this strength of Raman microspectroscopy.

Code: FOA	Time Slot/Poster Number: 09:30 - 9:50 am	Session: Raman Imaging III
Non-Invasive Imaging of Modified Liposomal Pharmaceutical Nanocarrier by Raman Microscopy
Tatyana Chernenko; Rupa Sawant; Vladimir Torchilin; Max Diem
Northeastern University, Boston, MA
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Summary
Nanosized drug delivery carriers represent an extensively growing field of pharmaceutical sciences. The optimal material for in vivo administrations should be biocompatible and biodegradable material. An important task with these materials is to follow their fate in situ, which require diverse labels or tags to be used for their detection. In combination with optical microscopy, Raman spectroscopy may obtain a biochemical image of a cell, which has been subjected to the action of various nanocarriers. A further advantage lies in the utilization of multivariate analyses which have the potential to differentiate between subcellular organelles and exogenous nanocarrier systems.

Code: FOA	Time Slot/Poster Number: 09:50 - 10:10 am	Session: Raman Imaging III
Vibrational Microspectroscopic Imaging: Applications to Skin Science and Wound Healing
Richard Mendelsohn; Carol Flach
Department of Chemistry, Rutgers University, Newark, NJ
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Summary
The current presentation will illustrate the utility of Raman and IR imaging spectroscopy and imaging in skin science. Three applications will be discussed as follows: 1) The feasibility of tracking and spatially imaging, in intact skin,the prodrug-to-drug interconversion of 5-fluorouracil, a well-known anti- cancer agent, will be demonstrated, 2) The kinetics of a solid-solid phase transition in intact skin tissue is utilized to track the reformation of the skin barrier in intact stratum corneum following a thermal perturbation, 3)Tracking the temporal expression and spatial distribution of various activated collagens and keratins during wound healing is demonstrated.

Code: FOA	Time Slot/Poster Number: 10:10 - 10:30 am	Session: Raman Imaging III
Advancing Raman molecular imaging for disease diagnosis
Shona Stewart; Amy Drauch; John Maier
ChemImage Corporation, Pittsburgh, PA
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Summary
Raman molecular imaging (RMI) is well suited to histopathological evaluation of tissue for disease diagnosis. Images exhibiting contrast due to highly specific Raman spectra at each pixel in a field of view provide additional molecular information that can be utilized for diagnoses of disease in cases where traditional histopathological methodologies are inconclusive.