Abstract Session Viewer

MP10

Code: MP10	Time Slot/Poster Number: 013	Session: Coherent Raman (CARS, FSRS, time-domain Raman etc.)
CARS Investigation of Plant Leaf Surfaces
Juergen Popp^{1, 2}; Nadine Vogler^{1, 2}; Benjamin Dietzek^{1, 2}; Denis Akimov²; Ina Anja Weißflog^{1, 2}
¹Friedrich-Schiller-University Jena, Jena, Germany; ²Institute of photonic technology Jena, Jena, Germany

View Abstract PDF
Summary
Epicuticular waxes are found on the outer surface of plant cuticles and difficult to study in vivo. CARS – as a Raman based technique – not only provides structural insight but also chemical information by imaging the spatial distribution of Raman-active vibrations. We present a comparative study using CARS and scanning electron microscopy (SEM) to characterize the structure of epicuticular waxes.

Code: MP10	Time Slot/Poster Number: 014	Session: Coherent Raman (CARS, FSRS, time-domain Raman etc.)
CARS and Raman microscopy of Alzheimer’s Brain Tissue
Annika Enejder¹; Lena Nyberg¹; Fredrik Svedberg¹; Jia-Yi Li²
¹Chalmers University of Technology, Göteborg, Sweden; ²Wallenberg Neuroscience Center, Lund University, Lund, Sweden

View Abstract PDF
Summary
With the combination of 2-photon fluorescence (2PF) microscopy of Amyloid-β peptides, Coherent Anti-Stokes Raman Scattering (CARS) microscopy of lipids and full spectral characterization by Raman microspectroscopy, we have mapped the molecular contents in human Alzheimer’s brain tissue. Our results indicate that the hallmarks of the disease are not limited to the accumulation of protein aggregates but include a complex cascade of molecular events. CARS and 2PF microscopy volume images of distributions of lipids and Amyloid-β peptides will be shown together with Raman microspectroscopy grids for a more detailed molecular characterization.

Code: MP10	Time Slot/Poster Number: 015	Session: Coherent Raman (CARS, FSRS, time-domain Raman etc.)
Exploring the Resonance Excitation Wavelength Dependence of Raman Lineshapes in Ultrafast Raman Loss Spectroscopy
Adithya Lakshmanna Yapamanu
Indian Institute of Science, Bangalore 560 012, In, Bangalore, India

View Abstract PDF
Summary
Stimulated Raman lineshapes vary from negative Lorentzians to positive Lorentzians through dispersive lineshapes as Raman pump wavelength is varied from off-resonance to resonance and again goes back to negative Lorentzian when the Raman pump wavelength is varied from resonance to above resonance.

Code: MP10	Time Slot/Poster Number: 016	Session: Coherent Raman (CARS, FSRS, time-domain Raman etc.)
Comparative Study On The Composition Of Brain Tissue By Nonlinear Microscopy
Tobias Meyer¹; Norbert Bergner¹; Benjamin Dietzek^{1, 2}; Christoph Krafft¹; Denis Akimov²; Bernd Romeike³; Rupert Reichart⁴; Rolf Kalff⁴; Jürgen Popp^{1, 2}
¹IPHT, Jena, Germany; ²Institue of Physical Chemistry, FSU, Jena, Germany; ³Department of Neuropathology, University Hospital, Jena, Germany; ⁴Clinic for Neurosurgery, University Hospital, Jena, Germany

View Abstract PDF
Summary
Histopathology of dissected tissue is the most important tool for the definitive diagnosis of encephalic diseases. A widespread standard in pathology is haematoxylin-eosine (HE) staining. But this technique has several limitations. However, the application of nonlinear and especially coherent anti stokes Raman scattering (CARS) microscopy offers the possibility to overcome these limits in neuropathology. In this study we investigated the composition and morphology of domestic pig brain tissue and cancerous human brain tissue from biopsies. In comparison to the established HE staining method similar results can be obtained by combining nonlinear imaging techniques.

Code: MP10	Time Slot/Poster Number: 017	Session: Coherent Raman (CARS, FSRS, time-domain Raman etc.)
Advances in understanding of coherent Raman scattering for diagnostic uses
michele marrocco
ENEA, Santa Maria di Galeria (RM), Italy

View Abstract PDF
Summary
The research conducted by the author in the field of non-linear coherent Raman techniques is here discussed in light of two main subjects. First of all, the attention is focused on the influence of the vibration-rotation coupling in simple molecules used as probes in experiments where vibrational coherent anti-Stokes Raman signals carry information on high temperatures. Secondly, it is explained how the modeling of coherent Raman microscopy is simplified according to two different approaches that avoid the complications of fully numerical solutions of the vectorial problem.

Code: MP10	Time Slot/Poster Number: 018	Session: Coherent Raman (CARS, FSRS, time-domain Raman etc.)
Influence of Non-Linear Parametric Wave Interaction on Multi-Component SRS and Energy Concentration Phenomenon
Nicholas Korniyenko
Kiev State Univercity, Kiev, Ukraine

View Abstract PDF
Summary
The present report describes the development of the theory of multicomponent SRS taking into account many Stokes and anti-Stockes components. The theory is based on the joint systems of nonlinear Maxwell's equations for multiple resonantly interacting waves and equations for the density matrix describing non-linear polarization for all waves and occupancies of the resonance states. Along with the stepped combination processes the entire set of four-photon resonant parametric processes are accounted for.

Code: MP10	Time Slot/Poster Number: 019	Session: Coherent Raman (CARS, FSRS, time-domain Raman etc.)
Imaging USPIO Nanoparticles Uptakes in Biological Systems with Vibrational Microspectroscopy
Lina Machtoub
Innsbruck Medical University, Innsbruck, Austria

View Abstract PDF
Summary
The scope of this work encompasses application of nonlinear optical microscopy to monitor the biodistribution of nanoparticles contrast agents and investigating their metabolic impact in neurodegenerative disorders systems. Experiments were performed on brain tissues, taken from a transgenic amyotrophic lateral sclerosis rat model, magnetically labelled with antibodies tagged with ultrasmall paramagnetic iron oxide nanoparticles (USPIO). Marked intensity enhancements have been observed in specific pathological regions of the ALS brain in compared with (WT) rat models. The results presented shows the promising potential of vibrational microspectroscopy in monitoring nanoparticles up-takes in neurodegenerative disorders model systems.

Code: MP10	Time Slot/Poster Number: 020	Session: Coherent Raman (CARS, FSRS, time-domain Raman etc.)
Monitoring Collagen Structures In Basal Cell Carcinoma Using Multimodal Imaging
Nadine Vogler^{1, 2}; Tobias Meyer¹; Katarina Svanberg³; Niels Bendsoe³; Benjamin Dietzek^{1, 2}; Jürgen Popp^{1, 2}
¹Institute of Photonic Technology, Jena, Germany; ²Friedrich-Schiller-University, Jena, Germany; ³Lund University Hospital, Lund, Sweden

View Abstract PDF
Summary
Our study combines linear Raman spectroscopy with nonlinear microscopic techniques, i.e. two-photon fluorescence, second-harmonic-generation and coherent anti-Stokes Raman (CARS) microscopy, to investigate the morphochemistry of a basal cell carcinoma.

Code: MP10	Time Slot/Poster Number: 022	Session: Coherent Raman (CARS, FSRS, time-domain Raman etc.)
Investigation of the Low Frequency Dynamics of Sulfur Ligated Heme Proteins
Karunakaran Venugopal; Paul Champion
Department of Physics, Northeastern University, Boston, MA

View Abstract PDF
Summary
Femtosecond vibrational coherence spectroscopy (VCS) is used to study the low frequency dynamics of cystathionine beta-synthase , cytochrome P450cam, and cytochrome c . A rich pattern of modes below 200 cm-1 is revealed in these proteins for the first time using the VCS technique.

Code: MP10	Time Slot/Poster Number: 023	Session: Coherent Raman (CARS, FSRS, time-domain Raman etc.)
Dynamic Process Measurements in the Complex Plane with Vibrational Phase Contrast CARS
Erik Garbacik; Martin Jurna; Jeroen Korterik; Cees Otto; Jennifer Herek; Herman Offerhaus
University of Twente, Enschede, Netherlands

View Abstract PDF
Summary
Measuring both the amplitude and phase of CARS signals allows us to increase the sensitivity of measurements by removal of the non-resonant background and enhance the chemical selectivity by allowing separation of molecular species whose vibrational spectra significantly overlap. With vibrational phase contrast CARS we have performed quantitative concentration measurements of homogeneous solutions of two resonant compounds and resonant/non-resonant combinations, as well as demonstrated chemically-selective imaging in a heterogeneous sample.

Code: MP10	Time Slot/Poster Number: 024	Session: Coherent Raman (CARS, FSRS, time-domain Raman etc.)
Hyperspectral Data Processing for Chemoselective MCARS Microscopy Using Principal Component Analysis
Christoph Pohling; Tiago Buckup; Marcus Motzkus
Universität Heidelberg, Heidelberg, Germany

View Abstract PDF
Summary
Multiplex Coherent Anti-Stokes Raman Scattering (MCARS) provides labeling free characterization of material and biological samples in nonlinear microscopy. ,We present a versatile algorithm for CARS microscopy that automatically provides chemoselective image contrast of CARS hyperspectral data sets. The whole spectral range is taken into account for image processing and no a priori information on the individual components of the sample is necessary.

Code: MP10	Time Slot/Poster Number: 025	Session: Coherent Raman (CARS, FSRS, time-domain Raman etc.)
Ultrafast Raman Loss Study of Excited State Evolution of Alpha-Terthiophene
Babita Mallick
Research Scholar, Bangalore, India

View Abstract PDF
Summary
The rapid data acquisition, natural fluorescence rejection and experimental ease institute Ultra-fast Raman loss scattering (URLS) as a unique valuable structure determining technique. Considering the advantages, URLS can be employed to understand structural dynamics and reactivity of ultrafast processes in chemical and biological systems. This is demonstated using alpha-Terthiophene, a thiophene based oligomers. In this study, following excitation to S1 state with a photo-pump (396 nm), the relaxation dynamics to S0 is studied using URLS.

Code: MP10	Time Slot/Poster Number: 026	Session: Coherent Raman (CARS, FSRS, time-domain Raman etc.)
Vibrational Coherence Spectroscopy Investigation of Cytochrome C Unfolding
Yuhan Sun; Karunakaran Venugopal; Abdelkrim Benabbas; Arthur McClelland; Paul Champion
Northeastern University, Boston, MA

View Abstract PDF
Summary
We studied low frequency vibrational modes of unfolded ferric cytochrome c in guanidine hydrochloride solutions using femtosecond vibrational coherence spectroscopy. A mode at ~ 77 cm-1, observed for fully unfolded cytochrome c at high guanidine hydrochloride concentration, is robust under all pH conditions measured. This suggests that heme geometric distortions in unfolded cytochrome c are different from those found in native cytochrome c.

Code: MP10	Time Slot/Poster Number: 027	Session: Coherent Raman (CARS, FSRS, time-domain Raman etc.)
CARS Microspectroscopic Study of Human Hair Using a Nanosecond White Light Laser Source
Kotatsu Bito¹; Shihomi Tokuhara¹; Satoru Naito¹; Yoshinori Masukawa¹; Masanari Okuno²; Hideaki Kano²; Hiro-o Hamaguchi^{2, 3}
¹Kao Corporation, Ichikai, Japan; ²The University of Tokyo, Bunkyo, Japan; ³National Chiao Tung University, Ta Hsueh Road, Taiwan

View Abstract PDF
Summary
We apply CARS microspectroscopy to human hair. The retrieved Im[chi(3)] spectra in the fingerprint region were obtained from human hair. Multicolor images of the hair sample were obtained by plotting the Im[chi(3)] intensity at each point. The amide III and S-O intensities are stronger in the cuticle(outer layer scales), in harmony with the facts that the secondary structure of cuticle proteins is mainly random and that cuticle proteins have a higher concentration of cysteic acid. The chemical and secondary structural distribution of proteins in human hair has thus been clearly visualized with our CARS system.

Code: MP10	Time Slot/Poster Number: 028	Session: Coherent Raman (CARS, FSRS, time-domain Raman etc.)
Polarization Sensitive CARS Investigations of Controlled Molecular Rotations
Laszlo Ujj¹; Ferenc Bartha²; Zengjun Chen¹; Chandra Prayaga¹; Timothy Royappa³; Charles Amos¹; Mauricio Tsukudaa ¹
¹Department of Physics, University of West Florida, Pensacola, Florida; ²University of Szeged, Szeged, Hungary; ³Department of Chemistry, UWF, Pensacola, Florida

View Abstract PDF
Summary
Polarization Sensitive Resonance Coherent anti-Stokes Raman Spectroscopy was applied to measure the vibrational manifold and reorientation of DCVJ upon external electric field for the first time. DCVJ is a charge transfer molecular rotor showing a viscosity dependent fluorescence quantum yield. This dependence is attributed to the “friction” type interaction between the 90 degree internal rotation of the molecule upon photo-excitation and the solvent cage surrounding it. Quantum chemical studies using DFT and normal mode calculations support our findings related to the conformational evolution of the molecule.

Code: MP10	Time Slot/Poster Number: 029	Session: Coherent Raman (CARS, FSRS, time-domain Raman etc.)
CARS at «Hard-to-Realize Conditions»: Lineshape Spectroscopy at High Temperatures in a Real Flame.
Konstantin Vereshchagin¹; Alexey Vereshchagin¹; Valery Smirnov¹; Oleg Stel’makh¹; Victor Fabelinsky¹; Walter Clauss²; Michael Oschwald²
¹General Physics Institute of Russian Academy of Sc, Moscow, Russian Federation; ²German Aerospace Center Space Propulsion Institute, Langer Grund, 74239, Hardthausen, Germany

View Abstract PDF
Summary
On the example of the study of the collisional broadening and shift of the hydrogen Q-branch lines due to collisions with water molecules in wide temperatures range [2000 K - 3000 K] we display the use of CARS for spectroscopic research of such hard-to-obtain objects, as a gas mixture of the predetermined composition at high temperatures in a real flame. In the presentation we discuss the basic peculiarities of single-shot lineshape spectroscopy and application of CARS for spectroscopic study of hard-to-obtain objects.