|
Code:
TP04
|
Time Slot/Poster Number:
154
|
Session:
Tip-Enhanced and Near-field Raman I
|
Tip Enhanced Raman Spectroscopy: Comparison Between Experiments and Numerical Simulations
|
| Victor Le Nader1; Jean-Yves Mevellec1; Tiberiu Minea2; Guy Louarn1
|
1Institut des Matériaux Jean Rouxel - UMR 6502, Nantes, France; 2Laboratoire de Physique des Gaz et Plasmas, Paris, France
|
|
| View Abstract PDF |
| Summary |
In this work we introduce an experimental TERS device developed in our laboratory dedicated to nano-object investigation. In particular, we describe how a commercial spectrometer has been associated with a tip-probe nano-positioning system. A numerical survey was performed to optimize the geometry of the device and to understand experimental results.
|
|
Code:
TP04
|
Time Slot/Poster Number:
155
|
Session:
Tip-Enhanced and Near-field Raman I
|
Probing Cell Membrane Models by Tip-Enhanced Raman Spectroscopy - TERS
|
| René Böhme1; Marc Richter2; Petra Rösch1; Volker Deckert1; Jürgen Popp1
|
1Friedrich Schiller University, Jena, Germany; 2Institute of Photonic Technologies, Jena, Germany
|
|
| View Abstract PDF |
| Summary |
Looking for a simple and robust model for cell membranes, supported lipid bilayers labeled with proteins were used and investigated by tip-enhanced Raman spectroscopy. Within this label free and non-destructive approach, a nanoscaled surface characterization was gained. Therefore, TERS spectra were classified, either typical for lipids, proteins or a mixture. Hence, a very first bio imaging procedure using near field features is shown, which provides single protein detection.
|
|
Code:
TP04
|
Time Slot/Poster Number:
156
|
Session:
Tip-Enhanced and Near-field Raman I
|
Describing Single Proteins Located In Membrane Structures by TERS
|
| René Böhme; Petra Rösch; Volker Deckert; Jürgen Popp
|
Friedrich Schiller University, Jena, Germany
|
|
| View Abstract PDF |
| Summary |
Cyt C molecules were investigated by tip-enhanced Raman spectroscopy (TERS). Adsorbed to mono- or double layer of lipid molecules, spectra of protein structures show specific features originated by the environment. Thus, an intresting approach of probing mitochondria like membranes is shwon. Due to the outstanding resolution of TERS, current investigations provide the characterization of several proteins down to the single molecule level.
|
|
Code:
TP04
|
Time Slot/Poster Number:
157
|
Session:
Tip-Enhanced and Near-field Raman I
|
Tip-enhanced Near-field Raman Spectroscopy of Graphite Thin Layers
|
| Yuika Saito1; Kyoko Masui2; Prabhat Verma2; Satoshi Kawata2
|
1Frontier Research Center, Osaka University, Osaka, Japan; 2Department of applied physics, Osaka University, Osaka, Japan
|
|
| View Abstract PDF |
| Summary |
We demonstrate the nano-scale optical analysis of graphene layers by tip-enhanced near-field Raman spectroscopy (TERS). In this technique, the spatial resolution ~30 nm is realized by the near-field probe which act as a nano-light source . TERS measurement across the graphene layer edges revels the nano-scale properties of the materials well as presenting the existence of local defects and edge boundaries, which is critical for the evaluation of this material especially in future device applications.
|
|
Code:
TP04
|
Time Slot/Poster Number:
158
|
Session:
Tip-Enhanced and Near-field Raman I
|
TERS in the sub-nanometric vicinity of a metallic tip
|
| Prabhat Verma; Taro Ichimura; Taka-aki Yano; Yuika Saito; Satoshi Kawata
|
Osaka University, Osaka, Japan
|
|
| View Abstract PDF |
| Summary |
Apart from the electromagnatic or plasmonic effects, the tip-sample interaction in TERS involves chemical and mechanical effects. Since the later two interaction mechanisms are effective for very short ranges, typically at molecular distances, it is not easy to separate them out in a TERS experiments. Here we demonstrate a sub-nanometric control over the tip-sample distance, and hence resolve the three interaction mechanisms. Utilizing the same techniques, we also show that super high-resolution imaging with a spatial resolution of about 3 nm could be possible.
|
|
Code:
TP04
|
Time Slot/Poster Number:
159
|
Session:
Tip-Enhanced and Near-field Raman I
|
Comparative study of Broadband CARS Techniques for Tip-enhanced Microscopy and Spectroscopy
|
| Kentaro Furusawa; Norihiko Hayazawa; Satoshi Kawata
|
Nanophotonics Laboratory, RIKEN, Wako, Japan
|
|
| View Abstract PDF |
| Summary |
We present the comparison of two-beam broadband CARS techniques based on photonic crystal fiber (PCF) and broadband oscillator sources, aiming at the improvements in spectral acquisition speed in tip-enhanced (TE) microscopy and spectroscopy. We show that they have different issues such as non-resonant background suppression and power handling. Although both are strongly dependent on the sample, a greater signal photon flux rate that can be achieved by using a PCF based approach seems more promising given a small local volume excited in TE-CARS. Preliminary results on TE-CARS experiments are also presented.
|
|
Code:
TP04
|
Time Slot/Poster Number:
160
|
Session:
Tip-Enhanced and Near-field Raman I
|
Tip-heating assisted TERS at elevated temperatures
|
| Alvarado Tarun1; Norihiko Hayazawa1, 3; Takaaki Yano2, 3; Satoshi Kawata1, 2
|
1The Institute of Physical and Chemical Research, Wako, Japan; 2Osaka University, Osaka, Japan; 3CREST, Japan Science and Technology Agency, Saitama, Japan
|
|
| View Abstract PDF |
| Summary |
In this work, we demonstrate heating-assisted TERS using the heat generated at the apex of a metallic-coated Si tip that is transferred to the CNT sample by conduction. The joule heat generated at the apex due the absorption of light, which is proportional to the imaginary part of the dielectric function from tip and sample[2], was used to thermally perturb and characterize a small volume in the CNT sample that is positioned underneath the tip. The dependence of TERS enhancement at highly elevated temperature was experimentally investigated and numerically studied using Finite Element Method (FEM).
|
|
Code:
TP04
|
Time Slot/Poster Number:
161
|
Session:
Tip-Enhanced and Near-field Raman I
|
Multivariate Analysis of TERS Maps on a Single Human Colon Cancer Cell
|
| Marc Richter1; Martin Hedegaard2; Tanja Deckert-Gaudig1; Volker Deckert1, 3
|
1IPHT - Institute of Photonic Technology, Jena, Germany; 2Institute of Sensors, Signals and Electrotechnics, Odensen, Denmark; 3Institute for Physical Chemistry, Jena, Germany
|
|
| View Abstract PDF |
| Summary |
Tip-enhanced Raman spectroscopy was used as non-destructive and label-free method for cell surface characterization. Using the hyperspectral unmixing algorithm N-FINDR a distribution map of proteins and lipids with nanometer resolution can be achieved by assigning TERS spectra to lipids, proteins or a mixture. This combination of methods enables a protein/lipid mapping with single protein resolution.
|