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Code:
TP08
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Time Slot/Poster Number:
125
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Session:
SERS applications I
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Monitoring Biomimetic lipid membrane compositions and structural changes in bound protein on biocompatible gold nanoparticles surfaces by vibrational spectroscopy
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| Tibebe Lemma; Robert J Foster; Tia E Keyes
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Dublin City University, Glasnevin, Ireland
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| View Abstract PDF |
| Summary |
Studies of mesoscopic models of lipid bilayers with embedded proteins on biocompatible gold nanoparticles surfaces, by Raman microscopy, infrared attenuated total reflection ( ATR) spectroscopy and Atomic force microscopy ( AFM).
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Code:
TP08
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Time Slot/Poster Number:
126
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Session:
SERS applications I
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Surface-enhanced Raman scattering (SERS) studies of Methyl orange based on AgBr colloids prepared by using electrolysis method
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| Minzhen Si1, 2; Renming Liu1; Yipu Kang1; Zhiguo Zhang2
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1Chuxiong Normal University, Chuxiong , China; 2Harbin Institute of Technology University, Harbin , China
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| View Abstract PDF |
| Summary |
A new SERS-active substrate of colloidal AgBr nanoparticles (AgBr CNPs) was prepared by using a simple method of electrolysis. By the SERS measurements of methyl orange (MO) based on the AgBr colloid, the AgBr colloid was shown to be an excellent SERS substrate for the detection of MO. Meanwhile, Raman frequencies of the MO molecule were calculated by using the method of DFT-B3LYP/6-31G*It was found that the DFT calculation of Raman spectrum matches well with the experimental results, whereas the SERS spectra did not appear with Ag colloids that were made by chemical reduction method.
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Code:
TP08
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Time Slot/Poster Number:
127
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Session:
SERS applications I
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The Optical Property Characterization of SERS-Encoded Nanoprobe.
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| Jinjoo Chae; Gunsung Kim; Homan Kang; Yoon-Sik Lee; Dae Hong Jeong
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Seoul National University, Seoul, South Korea
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| View Abstract PDF |
| Summary |
Surface-enhanced Raman Scattering (SERS) has been intensively studied to find its applications for ultra-sensitive biomolecule detection system. However, for practical applications of SERS-encoded nanoprobes, it is necessary to characterize them in respect of signal stability, reproducibility and so on. For example, SERS enhancement factor ranges from 10^4 to 10^14 by the size, shape and chemical composition of metal nanoparticle, SERS intensity is intrinsically heterogeneous hindering its application to quantification.
Previously, our group reported silica-based SERS-encoded nanoprobes called SERS Dot, as basic research for the quantitative application, we characterized optical properties of the SERS-encoded nanoprobe.
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Code:
TP08
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Time Slot/Poster Number:
128
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Session:
SERS applications I
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Nanoaggregate Embedded Beads as SERS Nanosensor for Multiplexed Pathogen Detection
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| Li-Lin Tay1; Ping-Ji Huang2; Lai-Kwan Chau2; Shannon Ryan1; John E. Hulse1; Jamshid Tanha1
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1National Research Council Canada, Ottawa, Canada; 2National Chung Cheng University, Chia-Yi, Taiwan
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| View Abstract PDF |
| Summary |
The detection of pathogenic bacteria bears important implications in both public health and security. Routine identification of bacteria involves biochemical characterization of cultured microorganisms taken from the contaminated sources. Clinical analysis requires a biomass of ~ 10^8 cells from the cultured medium for biochemical assay. Pathogen screening by means of surface enhanced Raman scattering (SERS) provides a faster and more sensitive alternative route towards the identification and detection of pathogenic bacteria. Here, we will outline the use of novel nanoaggregate embedded beads (NAEB) as ultra-sensitive SERS nanosensors for multiplexed detection of food borne pathogens, Staphylococcus aureus and Salmonella typhimurium.
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Code:
TP08
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Time Slot/Poster Number:
129
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Session:
SERS applications I
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Metal core Organosilica shell Multifunctional Nanoparticles for Multimodal Cell Imaging
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| Yan Cui; Bin Ren; Zhong-Qun Tian
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Xiamen University, Xiamen, China
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| View Abstract PDF |
| Summary |
Metal core organosilica nanoparticles were synthesized by the combination of the special optical property of metal core and easy functionalization of organosilia shell. This method was further used to synthesize multifunctional nanoparticles with Raman reporter adsorbed directly on the surface and the fluorescence molecule embedded within organosilica shell. The multifunctional nanoparticles exhibiting SERS, Rayleigh scattering and fluorescence signals can be used as multimodal tracer with potential application in the immunoassay analysis, living cell imaging and related application.
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Code:
TP08
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Time Slot/Poster Number:
130
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Session:
SERS applications I
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SERS of the New Rare-Earth-Acenocoumarol Complexes
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| Andronie Luisa
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univ.babes-bolyai, Cluj-Napoca, Romania
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| View Abstract PDF |
| Summary |
Incorporation of lanthanides into coordination compounds as potential medicinal agents, both therapeutic and diagnostic, is the most recent development that is yielding rich rewards [1]. Here we report the predicted structural characterization of the new prepared Dy- and La-acenocoumarol (4-hydroxy-3[1-(4-nitrophenyl)-3-oxobutyl]-2H-1-benzopyran-2-one) complexes supported by the vibrational analysis. Furthermore we applied here surface enhanced Raman scattering (SERS) in order to evaluate the possibility of the technique to detect trace amounts of the rare-earth-acenocoumarol complexes and to investigate the adsorption behaviour of the complex to the Ag colloidal nanoparticles.
REFERENCES
1. Katherine H. Thompson and Chris Orvig, Chem. Soc. Rev., 35, 499 (2006).
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Code:
TP08
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Time Slot/Poster Number:
131
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Session:
SERS applications I
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A Combined SERR Spectroscopic And Electrochemical Approach To Study Enzymes On Electrodes
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| Murat Sezer1; Diego Millo1; Roberto Spricigo2; Ulla Wollenberger2; Tillmann Utesch1; M. Andrea Mroginski1; Peter Hildebrandt1; Inez M. Weidinger1
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1Technische Universität Berlin, Institut für Chemie, Straße des 17. Juni 135, 10623 Berlin, Germany; 2Universität Potsdam, Inst. f. Biochem. u. Biologie, Karl-Liebknecht Straße 24-25, H. 25, 14476 Golm , Germany
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| View Abstract PDF |
| Summary |
Surface enhanced resonance Raman (SERR) spectroscopic and electrochemical measurements were performed to study human Sulfite Oxidase (hSO) immobilized on nanostructured silver electrodes coated by a self-assembled monolayer (SAM) of marcapatanes. Upon immobilization an electrical communication between the Cyt b5 domain and the electrode could be achieved such that it was possible to monitor the catalytic current under turnover conditions. SERR spectroscopy yielded detailed information about the redox state and structure of the heme center in the Cyt b5 domain. The study of hSO was supplemented by the investigation of the isolated Cyt b5 domain.
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Code:
TP08
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Time Slot/Poster Number:
132
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Session:
SERS applications I
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Shell-Isolated Nanoparticle-Enhanced Raman Spectroscopy for Inspecting Pesticide Residues
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| Jian-Feng Li; Song-Bo Li; Yi-Fan Huang; Bin Ren; Zhong-Qun Tian
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State Key Lab. of Phys. Chem. of Solid Surfaces, Xiamen, China
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| View Abstract PDF |
| Summary |
We proposed a new generic strategy of using chemically inert shell-isolated nanoparticles to obtain surface Raman signals with high sensitivity from various materials and surface species, which could be used in inspecting food safety, drug, security, and environment protection rapidly.
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Code:
TP08
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Time Slot/Poster Number:
134
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Session:
SERS applications I
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Optimizing the SERS activity of 1,10 Phenanthroline molecule adsorbed on Au@Ag core-shell nano colloid
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| Joydeep Chowdhury1, 1; Tarasankar Pal2
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1Department of Physics, Sammilani Mahavidyalaya, Kolkata, India; 2Department of Chemistry, IIT, Kharagpur, India
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| View Abstract PDF |
| Summary |
Surface enhanced Raman scattering (SERS) has become an increasingly popular technique not only for studying the molecules or ions at trace concentrations but also in estimating their possible orientations on the nano structured metal surfaces. Here we report the variation of SER intensities of 1.0 x 10-9 M 1, 10 phenanthroline (PHEN) molecule adsorbed on Au @Ag core- shell nano colloids for fixed gold core and variable silver shell ratios. Finite difference time domain (FDTD) simulations were employed to explain the experimental results.
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Code:
TP08
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Time Slot/Poster Number:
135
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Session:
SERS applications I
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Highly Sensitive Clinical Diagnosis on Novel Gold-Array Chip Using Surface-Enhanced Raman Scattering Imaging
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| Moonkwon Lee; Sangyeop Lee; Hyangah Chon; Jaebum Choo
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Hanyang University, Ansan, South Korea
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| View Abstract PDF |
| Summary |
This paper reports a novel method for the highly sensitive cancer marker diagnosis using a gold-patterned microarray chip and a surface-enhanced Raman scattering imaging (SERSi). In this work, we designed and fabricated a conceptually new gold-patterned glass microarray chip. Here, gold patterned hydrophilic spots are completely isolated from hydrophobic surfaces, and the hydrophobic/hydrophilic hybrid property of the microarrays greatly improves the biomolecule deposition, spot uniformity, replication, and reproducibility. Also, SERSi technique is compensate the reliability problem in conventional SERS detection method. This novel gold-array chip and SERSi technique have been successfully used for a highly sensitive clinical diagnosis.
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Code:
TP08
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Time Slot/Poster Number:
136
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Session:
SERS applications I
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SERS Quantification of Entacapone Isomers
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| Marina Markovic; Tomislav Biljan
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PLIVA Croatia Ltd., Research and Development, Zagreb, Croatia
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| View Abstract PDF |
| Summary |
Raman spectroscopy has found widespread use in pharmaceutical applications. Here we report quantification of isomer mixture of an active pharmaceutical substance by SERS.
Entacapone is an active substance used in treatment of Parkinson’s disease. It exists in two isomeric forms Z and E , both showings good SERS spectra, using colloidal silver substrate. The E isomer has a higher affinity for the silver substrate. Mentioned difference gives an opportunity for SERS quantification of the isomer mixture.
Successful quantification of isomer mixtures in between 0 and 100% w/w has been performed using SERS technique, which is suitable for in-line reaction monitoring.
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Code:
TP08
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Time Slot/Poster Number:
137
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Session:
SERS applications I
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Adsorption Of Rhodanine Derivatives On Silver And Gold Nanoparticle Surfaces
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| Kamilla Malek; Katarzyna Marzec; Krzysztof Gebski; Agnieszka Kaczor
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Faculty of Chemistry, Jagiellonian University, Krakow, Poland
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| View Abstract PDF |
| Summary |
In this work we study adsorption mechanism of benzylidene rhodanines on silver and gold nanocolloidal particles by using Surface Enhancement Raman Spectroscopy. The comparison of normal and SER spectra of all the compounds shows enhancement of bands that originate from the stretching modes of the phenyl and heterocyclic rings upon interaction with metal surfaces. The differences between SER spectra collected on silver and gold colloids are negligible.
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Code:
TP08
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Time Slot/Poster Number:
138
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Session:
SERS applications I
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Raman Spectroscopic Investigation of Dyes in Spices
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| Ute Uhlemann1; Petra Rösch1; Anuradha Ramoji1; Paulo Augusto Da Costa Filho2; Fabien Robert2; Jürgen Popp1, 3
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1Friedrich Schiller University, Jena, Germany; 2Nestlé Research Center, Lausanne, Switzerland; 3Institute of Photonic Technology, Jena, Germany
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| View Abstract PDF |
| Summary |
In this study, a number of synthetic colorants for spices have been investigated by means of Raman spectroscopy, resonance Raman spectroscopy, and surface enhanced (resonance) Raman spectroscopy (SER(R)S). The aim of the study was the determination of limits of detection for each dye separately and in binary mixtures of dyes in spiked samples of the spices.
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Code:
TP08
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Time Slot/Poster Number:
139
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Session:
SERS applications I
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SERS and Solid Phase Synthesis
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| Magdalena Gellner1; Hannes Kuchelmeister2; Carsten Schmuck2; Sebastian Schlücker1
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1University of Osnabrück, Osnabrück, Germany; 2University of Duisburg-Essen, Duisburg-Essen, Germany
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| View Abstract PDF |
| Summary |
Solid-phase synthesis is an important tool for the preparation of DNA, peptides and carbohydrates as well as compounds used in supramolecular and medicinal chemistry. Usually, the solid support is a modified polystyrene resin with a loading of 100 pmol per bead. Thus, the analysis of solid-phase-bound compounds directly on a single bead poses a challenge. We recently demonstrated the direct, label-free and surface-selective detection of solid-phase-bound compounds via SERS.
In this contribution we show an integrated approach without the need for adding nanoparticles externally: both solid support and the SERS-active substrate are combined into a single functional unit.
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Code:
TP08
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Time Slot/Poster Number:
140
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Session:
SERS applications I
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Potential-dependent Characterization of Bombesin Adsorbed on Roughened Ag, Au, and Cu Electrode Surfaces
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| Edyta Podstawka1; Gediminas Niaura2; Katarzyna M. Marzec1; Leonard Proniewicz1; Younkyoo Kim3
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1Faculty of Chemistry, Jagiellonian University, Krakow, Poland; 2Institute of Biochemistry, Vilnius, Lithuania; 3Hankuk University of Foreign Studies, Yongin, Korea
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| View Abstract PDF |
| Summary |
The variation among individual cancers is usually affected by complex interactions between carcinogens and the host genome. One of the host genome agents is bombesin (BN, pGlu-Gln-Arg-Leu-Gly-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH2). This work reports the direct surface-enhanced Raman spectroscopic (SERS) and Generalized Two-Dimensional Correlation Analysis (G2DCA) observations of the different orientations of BN chemisorbed on electrochemically roughened Ag, Au, and Cu electrode surfaces at different applied electrode potentials and at physiological pH.
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Code:
TP08
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Time Slot/Poster Number:
141
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Session:
SERS applications I
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Direct Silica Encapsulation of SERS Labels via SAMs Containing Terminal SiO2 Precursors
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| Max Schütz1; Bernd Küstner1; Manuel Bauer2; Carsten Schmuck2; Sebastian Schlücker1
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1University of Osnabrück, Osnabrück, Germany; 2University of Duisburg-Essen, Essen, Germany
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| View Abstract PDF |
| Summary |
Functionalized metal nanoparticles can be used for the selective localization of biomolecules such as proteins in cells and tissue specimens with SERS as the read-out method (SERS microscopy). Here we present a direct and fast route to SERS labels, comprising silica-encapsulated self-assembled monolayers (SAMs) of Raman reporter molecules on Au/Ag nanoshells. The surface of the nanoparticles is rendered vitreophilic by using modified mercaptobenzoic acid derivatives containing a terminal SiO2 precursor. The SAM of these modified Raman reporter molecules provides an intense SERS signal and the terminal SiO2 precursors render the surface vitreophilic for a direct silica-encapsulation.
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Code:
TP08
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Time Slot/Poster Number:
142
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Session:
SERS applications I
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A New Nanosensor For Polycyclic Aromatic Hydrocarbons Detection In Marine Environment
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| Nene Thiune1; Catalina David1; Salem BEN-AMOR1; Nicolas Guillot1; Hong SHEN 2; Olivier PERON 3; Emmanuel RINNERT3; Timothée Toury2; Marc Lamy de la Chapelle1
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1University Paris 13, Bobigny, France; 2Université de technologie de Troyes, 10010 Troyes, France; 3IFREMER, 29280 Plouzané, France
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| View Abstract PDF |
| Summary |
In this work we report an accurate synthesis of a SERS nanosensor based on nanoscale gold structures obtained by Electron Beam Lithography, suitable for environmental analysis.
The aim of this sensor is to detect Polycyclic Aromatic Hydrocarbons in marine environment at very low concentrations. Our first results strongly suggest that SERS intensity of naphthalene depends on gold nanostructures, prepared for example from colloid solutions. Indeed strong SERS intensities are measured on nanocylinder structures obtained by EBL. In addition, using a specific monoclonal antibody functionalised on gold, our SERS nanosensors exhibit a strong specificity for PAH detection.
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Code:
TP08
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Time Slot/Poster Number:
143
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Session:
SERS applications I
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Use Of Hydrophilic Polymer For Reproducible Surface Enhanced Raman Optical Activity Spectroscopy (SEROA)
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| Saeideh Ostovar Pour1; Steven Bell2; Ewan Blanch3
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1PhD student, Manchester, United Kingdom; 2Director of Research, Belfast, UK; 3Senior Lecturer, Manchester, UK
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| View Abstract PDF |
| Summary |
As results of its sensitivity to chirality, Raman Optical Activity is powerful probe of the structure and behaviour of biomolecules in aqueous solution. The conditions of high concentration and long data collection time required for ROA limits its application, the combination of ROA with SERS in the form of SEROA could be a solution for widening the application of ROA. The generation of reliable SEROA spectra of biomolecules has been problematic due to non-homogenous colloidal systems forming. In order to prove the SEROA technique, D- and L-ribose have been measured in the presence of silver citrate-reduced colloids and polycarbophil polymer.
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Code:
TP08
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Time Slot/Poster Number:
145
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Session:
SERS applications I
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Detection of Anthrax Biomarker and Fentanyl using Ag film Substrate with Highly Reproducibility and Sensitivity by Surface-Enhanced Raman Scattering
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| Lingce Kong1; Guomin Zuo1; Guangqiang Liu2; Weiping Cai2; Zhenxing Cheng1
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1Institute of chemical defense, Beijing, China, Beijing, China; 2Institute of Solid State Physics, Chinese Academia, Hefei, Anhui
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| View Abstract PDF |
| Summary |
A silver film substrate with highly reproducibility and SERS activity was fabricated based on a chemical-bath-deposition method. The effects of deposition time of silver films on morphology, optical properties and SERS activity were investigated by FE-SEM, UV-vis and Raman spectroscopy, respectively. Then, the optimized silver films were used for detecting trace anthrax biomarker-dipicolinic acid (DPA) and an incapacitant-fentanyl. The limits of detection for DPA and fentanyl achieved 60 μM and 150 μM, respectively within 20 minutes.
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Code:
TP08
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Time Slot/Poster Number:
146
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Session:
SERS applications I
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SERS Detection in Capillary Separation Systems
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| Carolina Carrillo-Carriòna1, 2; Bartolomé M. Simonet2; Miguel Valcárcel2; Bernhard Lendl1
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1Vienna University of Technology, Institute of chem, Vienna, Austria; 2University of Cordoba, Cordoba, Spain
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| View Abstract PDF |
| Summary |
There is interest in the development of highly sensitive, molecular specific detection techniques in miniaturised separation systems such as capillary based liquid chromatography. Surface enhanced Raman scattering (SERS) is a promising detection technique but in order to be truly useful, a reliable analysis system must be achieved. Without such robustness SERS detection will not gain widespread acceptance. Here we report a novel colloidal synthesis method where hydroxylamine based silver colloids are formed in the presence of CdSe/ZnS Quantum Dots (QDs). The QDs act as a co-reducer and form a link separator between the colloid particles establishing more controlled enhancement.
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Code:
TP08
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Time Slot/Poster Number:
147
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Session:
SERS applications I
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Raman and SERS Investigation of the Photodegradation Process of Janus Green B in different pH values
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| Fabiana Inoue; Paola Corio
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Chemistry Department, University of São Paulo, São Paulo, Brazil
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| View Abstract PDF |
| Summary |
We investigate the photocatalytic degradation mechanism of the dye Janus Green in aqueous TiO2 suspension in different pH values by surface-enhanced Raman spectroscopy (SERS) for the analysis of the solution phase and by FT-Raman to the analysis of the catalyst surface as a function of UV irradiation. Changes in Raman spectra of catalyst surface and bulk solution during photocatalytic degradation suggest that intermediates formed at the semiconductor/water interface differ from those formed in aqueous solution, and also depend on pH. In addition, it can be suggested that the beginning of photocatalytic process occurs in different phases depending on pH value.
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Code:
TP08
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Time Slot/Poster Number:
148
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Session:
SERS applications I
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Application of SERS Immunoassay for Biosensing
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| H. K. Seo; W. J. Hong; D. H. Kang; S. R. Ryu; Y. M. Jung
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Kangwon National University, Chuncheon, Korea
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| View Abstract PDF |
| Summary |
SERS has been a promising candidate for various applications in chemical-biochemical analysis, nanostructure characterization and biomedical applications. Especially, SERS immunoassays based on antigen-antibody bonding have been studied for biomarkers for early cancer diagnosis.
To demonstrate the possible application of SERS immunoassay for detection of antigen (PSA) in cancer cells, we performed the sandwich type SERS immoassay process. We prepared a monolayer of gold nanoparticles on the 3-aminopropyl trimethoxysilane (APTMS)-derivatized surface of a glass slide by using the self-assenbled monolayer technique, to capture antigen onto the immobilized layer of antibodies on the gold substrate.
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