Code: ThOA	Time Slot/Poster Number: 10:30 - 10:50 am	Session: SERS Applications II
Controlled SERRS Using Biologically Driven Nanoparticle Assembly
Duncan Graham; Karen Faulds; David Thompson; Fiona McKenzie; Ross Stevenson
University of Strathclyde, Glasgow, United Kingdom
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Summary
Metallic nanoparticles can be used as basic materials for a wide variety of purposes including building blocks for nanoassemblies, substrates for enhanced spectroscopies such as fluorescence and Raman and as labels for biomolecules. Here we report how silver and gold nanoparticles can be functionalised with specific biomolecular probes to interact in a specific manner with a target molecule to provide a change in the properties of the nanoparticles which can be measured to indicate the molecular recognition event. This concept is demonstrated by DNA hybridisation using different sequences, types of nanoparticles and orientations.

Code: ThOA	Time Slot/Poster Number: 10:50 - 11:10 am	Session: SERS Applications II
Quantifying Resonant Raman Cross Sections With SERS
Stefan Andreas Meyer; Eric C. Le Ru; Pablo G. Etchegoin
Victoria University of Wellington, Wellington, New Zealand
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Summary
We propose a method based on surface-enhanced Raman scattering (SERS) to estimate the resonance Raman cross sections of dyes. The latter are notoriously difficult (or impossible) to obtain by normal (spontaneous) constant wave Raman spectroscopy when the fluorescence quantum yield of the molecules is good and the overwhelming effect of fluorescence masks the Raman spectrum. We propose here to use the fluorescence quenching occurring in SERS conditions to overcome simply this problem. The method is demonstrated by estimating the resonance Raman differential cross sections for Rhodamine 6G for seven different excitation wavelengths across the visible range.

Code: ThOA	Time Slot/Poster Number: 11:10 - 11:30 am	Session: SERS Applications II
Immuno-SERS Microscopy: Nanoparticle Probes And Tissue Diagnostics
Mohammad Salehi; Max Schütz; Sebastian Schlücker
University of Osnabrueck, Osnabrueck, Germany
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Summary
SERS microscopy (µSERS) employs target-specific colloidal SERS probes in combination with Raman microspectroscopy. For example, SERS-labeled antibodies allow the selective and sensitive localization of the corresponding antigen in tissue specimens. Stability and robustness, sensitivity as well as steric accessibility for bioconjugation are few very important properties for SERS probes. Various designs for SERS lables including strategies for their chemical preparation are shown. Further, the applications of µSERS for the selective and sensitive localization of the tumor suppressor p63 in the benign prostate is demonstrated. Future directions of this innovative Raman/SERS microspectrosopic technique for tumor diagnostics will be discussed.

Code: ThOA	Time Slot/Poster Number: 11:30 - 11:50 am	Session: SERS Applications II
Towards an analytical tool based on Lab-on-a- Chip-SERS (LOC-SERS) for detection of drugs in complex matrices
Anne März1; Thomas Bocklitz1; Thomas Henkel3; Petra Rösch1; Michael Kiehntopf2; Jürgen Popp1
1FSU Jena, Institute of Physical Chemistry, Jena, Germany; 2Dept.of Clinical Chemistry&Laboratory Diagnostics, Jena, Germany; 3Institute of Photonic Technology (IPHT) Jena, Jena, Germany
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Summary
This contribution will show the great potential of lab-on-a-chip SERS (LOC-SERS) for the quantitative detection of drugs. The combination of SERS and a microfluidic device leads to an improve of reproducibility of quantitative SERS measurements and towards an analytical tool for the online detection of drugs in complex matrices.

Code: ThOA	Time Slot/Poster Number: 11:50 am - 12:10 pm	Session: SERS Applications II
Vibrational Characterization and Surface-Enhanced Raman Scattering Detection of Beta-Agonists used in Sport Doping
Santiago Sanchez-Cortes; Irene Izquierdo-Lorenzo; Jose Vicente Garcia-Ramos; Concepción Domingo
Instituto de Estructura de la Materia (CSIC), Madrid, Spain
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Summary
A vibrational characterization of beta-agonists clenbuterol, salbutamol and terbutaline by means of IR and Raman spectroscopy supported by ab initio DFT calculations is presented in this work. Surface-enhanced Raman scattering (SERS) spectra of these molecules on gold colloid are reported for the first time and proposed as an alternative method for the univocal detection of these drugs in anti-doping control at trace concentrations. A study of the affinity of the different molecules towards the nanoparticle surface depending on the pH of the medium is performed in order to optimize the SERS signal.

Code: ThOA	Time Slot/Poster Number: 12:10 - 12:30 pm	Session: SERS Applications II
Fingerprints….. fingerprinted by SERS!
Richard Spragg1; Leesa Ferguson2; Rosalind Wolstenholme2; Louis Tisinger1; Enrique Lozano Diz1
1PerkinElmer, Seer Green, United Kingdom; 2Sheffield Hallam University, City Campus, Sheffield
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Summary
The use of Surface Enhanced Raman Spectroscopy (SERS) is proposed here because, unlike some of the techniques previously reported, it has potential for analysis that is non-destructive and portable, and furthermore introduces spectroscopic signature to the samples identification. Using a PerkinElmer RamanStation 400 a study has been carried out to identify the feasibility of using different commercially available nanoparticles and it has been demonstrated that a gold colloid layer can be deposited such that the fingerprint is still visible but sufficient enhancement is achieved to record a spectrum of the print.