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ThOE

Code: ThOE	Time Slot/Poster Number: 2:00 - 2:30 pm	Session: Biomolecules & Living Systems I
Elucidating Viral Protein Structures and Assembly Mechanisms by Raman Spectroscopy
George J. Thomas
University of Missouri - Kansas City, Kansas City, MO

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Summary
Morphogenesis of icosahedral viruses involves the initial assembly of a precursor shell from a small number of virally encoded proteins, including the principal protein component of the shell. Structural details of precursor shells and transformation into the mature virion architecture are rigorously controlled along the in-vivo assembly pathway. Efficient assembly usually involves, in addition to constituent proteins of the shell, a virally encoded scaffolding protein to ensure the appropriate shell dimensions and assembly rate. The use of Raman spectroscopy to probe structures, molecular mechanisms and thermodynamics of assembly of the DNA bacterial viruses P22 and HK97 will be described.

Code: ThOE	Time Slot/Poster Number: 2:30 - 3:00 pm	Session: Biomolecules & Living Systems I
Deca-second Mitochondria Dynamics of Living Yeast Cells as Revealed by In Vivo Time- and Space-resolved Raman Spectroscopy
Mana Kato¹; Chikao Onogi¹; Hiro-o Hamaguchi^{1, 2}
¹University of Tokyo, Tokyo, Japan; ²National Chiao Tung University, Hsinchu, Taiwan

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Summary
The "Raman spectroscopic signature of life", which is a Raman band at 1602 cm-1 observed from mitochondria of living yeast cells and which sharply reflects their metabolic activity, has been found to show marked intensity variations with a few deca-second time period. This observation is most likely to be related with the fast mitochondria dynamics known as respiration oscillation.

Code: ThOE	Time Slot/Poster Number: 3:00 - 3:30 pm	Session: Biomolecules & Living Systems I
Laser Tweezers Raman Spectroscopic Analysis of Single Cells and Their Dynamics
James Chan^{1, 2}; Tobias Moritz^{2, 3}; Douglas Taylor^{2, 4}; Christopher Polage^{2, 5}; Denise Krol^{2, 6}; Stephen Lane²
¹Lawrence Livermore National Laboratory, Livermore, CA; ²NSF Center for Biophotonics Science and Technology, Sacramento, CA; ³Biophysics Graduate Group, UC Davis, Davis, CA; ⁴Dept of Pediatrics, UC Davis Medical Center, Sacramento, CA; ⁵Dept of Pathology, UC Davis Medical Center, Sacramento, CA; ⁶Dept of Applied Science, UC Davis, Davis, CA

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Summary
Laser tweezers Raman spectroscopy (LTRS) has emerged as a promising Raman approach for the noninvasive analysis of single biological cells in solution and has many potential biomedical applications. Here, we apply LTRS for monitoring cellular drug response and demonstrate, using microbial and cancer cells, the feasibility of this approach to detect spectral changes of individual cells as they interact with the drugs over time. These studies have important clinical implications for developing LTRS as a new method for rapid testing and identification of infectious diseases or for in-vitro monitoring of patient response to chemotherapy.

Code: ThOE	Time Slot/Poster Number: 3:30 - 3:50 pm	Session: Biomolecules & Living Systems I
Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy
Anna Chiara De Luca¹; Michael Mazilu¹; Andrew Riches²; Simon Herrington²; Kishan Dholakia¹
¹SUPA, Univeristy of St Andrews, St Andrews, United Kingdom; ²Bute Medical School, Univeristy of St Andrews, St Andrews, UK

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Summary
A new modulation method for separating Raman scattering from fluorescence has been developed that uses the principle of multi-channel lock-in detection. The method allows a suppression of the fluorescent background and improves spectral quality of the Raman data. It can remove in real-time static background and render visible weak Raman features that are masked by the fluorescence in the standard spectrum. In this work, we show that our modulation method provides, with high efficiency, Raman spectra of different locations (nucleus, cytoplasm and membrane) within a single cell, suggesting that this minimally invasive optical technology has potential for bio-medical diagnosis.

Code: ThOE	Time Slot/Poster Number: 3:50 - 4:10 pm	Session: Biomolecules & Living Systems I
Surface-enhanced Raman spectroscopic detection of human CaSki cells using clean and uniform substrates of assembled Au nanoparticles
Jiayi Huang; Yanhui Xu; Bin Ren; Zhongqun Tian
Xiamen University, Xiamen, China

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Summary
In order to achieve early diagnosis of cervical cancer, surface enhanced Raman spectroscopy (SERS) was proposed to study the biochemical composition and structure of the membrane of CaSki cells, a common cervical cancer cells. The result shows that SERS is a very powerful technique for identifying the living cells in different states.