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Code:
ThP02
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Time Slot/Poster Number:
001
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Session:
Biological Cells and Tissues
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In Situ Measurement of Astaxanthin In Biological Material
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| Agnieszka Kaczor; Malgorzata Baranska
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Faculty of Chemistry, Jagiellonian University, Krakow, Poland
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| View Abstract PDF |
| Summary |
Astaxanthin (AX) is a red-orange carotenoid pigment found in a wide range of aquatic animals, such as salmon, trout, shrimp, red seabream, lobster or fish eggs. AX is an unusually powerful antioxidant, with a high capacity of protection against life-style diseases, such as cancer, diabetes and some immunological system ailments.
Two-dimensional Raman maps of parts of aquatic animals were obtained with the application of FT-Raman spectrometer (1064 nm) with the spatial resolution of 50-200*10-6 m and analyzed with the aid of quantum-chemistry calculations. The obtained results are compared, discussed and related to the molecular structure of AX.
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Code:
ThP02
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Time Slot/Poster Number:
002
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Session:
Biological Cells and Tissues
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Label-Free Non-Destructive Identification of
Stem Cells in the Hair Follicle with Confocal
Raman Spectrocopy
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| Katherine Lau1, 2; Christian Matthaeus1; Juergen Popp1, 2; Bayden Wood3; Jennifer Kloepper5; Ralf Paus4, 5; Volker Deckert1, 2
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1Institute of Photonic Technology, Jena, Germany; 2Institute for Physical Chemistry, FSU, Jena, Germany; 3Monash University, Victoria, Australia; 4University of Manchester, Manchester, UK; 5University of Luebeck, Luebeck, Germany
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| View Abstract PDF |
| Summary |
A confocal Raman system (WiTec, Germany) was employed to collect Raman spectra over a predefined map area within the dermal papilla of a hair follicle. The purpose of this study is to collect Raman spectra and to perform chemometric analysis in order to identify cell spectra which may belong to stem cells.
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Code:
ThP02
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Time Slot/Poster Number:
003
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Session:
Biological Cells and Tissues
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In-situ Raman spectroscopic imaging of a mussel coating and adhesive
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| Admir Masic1; Matthew Harrington1; J. Herbert Waite2; Peter Fratzl1
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1Max Planck Institute of Colloids and Interfaces, Potsdam, Germany; 2University of California, Santa Barbara, Santa Barbara, CA
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| View Abstract PDF |
| Summary |
Here we report in-situ high-resolution Raman spectroscopic imaging of the byssal coating and plaque showing micron level spatial distribution of various mussel foot proteins (mfp) and their interaction with Fe3+ using specific Resonance Raman spectral features. Results suggest that mfp-2, only one containing phenylalanine in its amino acid sequence, is mainly distributed in foam part of the plaque. Furthermore, we show that both mfp-1 (coating) and mfp-2 (foam) are capable of coordinating Fe(III), but the mussel carefully controls the coordination environment during processing.
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Code:
ThP02
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Time Slot/Poster Number:
004
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Session:
Biological Cells and Tissues
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Raman Microscopic Studies of Tissues and Cells
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| Samir El-Mashtoly1; Laven Mavarani1; Andrea Tannapfel2; Carsten Kötting1; Klaus Gerwert1
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1Lehrstuhl für Biophysik, Ruhr-Universität Bochum, Bochum, Germany; 2Institut für Pathologie, Ruhr-Universität Bochum, Bochum, Germany
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| View Abstract PDF |
| Summary |
Raman spectroscopy is becoming an increasingly applied technique in biomedical spectroscopy. This technique provides spatially resolved information on the basis of chemical composition of the different structural compartments. Raman microscopy is exceptionally well suited for differentiating distinct tissue structures and for identifying tissue pathology. Furthermore, Raman microscopy can be used for the identification of subcellular components of single bacterial cells. We report Raman spectral imaging of intestinal mucosa in order to identify spectral biomarkers which can discriminate between normal and cancerous mucosa. We also performed Raman measurements of Halobacterium salinarum cells to identify its components.
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Code:
ThP02
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Time Slot/Poster Number:
005
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Session:
Biological Cells and Tissues
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Evaluation of the effects of the
ultra-violet radiation of Antarctica on bovine corneas and lenses by Raman spectroscopy
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| Tatsuyuki Yamamoto1; Satoshi Imura2; Naoyuki Yamamoto3
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1Faculty of Life and Environmental Science, Shimane, Matsue, Japan; 2National Institute of Polar Research, 10-3 Tachikawa-shi, Tokyo 190-8518, Japan; 3Nagoya University, Nagoya 464-8601, Japan
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| View Abstract PDF |
| Summary |
The Raman spectra of bovine corneas and lenses irradiated to the ultra violet radiation at Syowa station of Antarctica were observed. The bovine crystallin occurred photo-induced cataract by the exposure to the solar radiation of mid-summer at Antarctica. Photo-induced decrease of Raman signals assigned to Trp residues suggests that the structural change of crystallin is correlated with the decomposition of them. The Raman spectra of the collagen of cornea showed little change, however FT-IR measurements showed that the IamideII／IamideI decreased much by the exposure to the solar radiation of mid-summer at Antarctica.
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Code:
ThP02
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Time Slot/Poster Number:
006
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Session:
Biological Cells and Tissues
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Raman Spectroscopic Characterization of Secondary Structure of Delta Crystallin Isolated from Mule Duck
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| Wenlung Chen; Chih-Hsien Wang
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Department of Applied Chemistry, National Chiayi U, Chiayi, Taiwan
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| View Abstract PDF |
| Summary |
Delta-crystallin, the major protein component of avian and reptilian, of which sequence is homologous to the enzyme of urea cycle – argininosuccinate lyase (ASL). The structure of delta-crystallin is different from that of ubiquitous crystallins such as alpha- and beta/gamma-crystallins. In this report, FT-Raman with near infrared excitation is employed to investigate delta-crystallin structure either at native state or denatured state arising from heat and chemical modifications (urea or SDS).
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Code:
ThP02
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Time Slot/Poster Number:
007
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Session:
Biological Cells and Tissues
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Bone Bio-Mineralization: in Depth Analysis of Hydroxylapatite Crystallization Through Experiments and Simulations
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| Barbara Pavan; Dan Zhou; Brandon Whitman; Marco Fornari; Mary Tecklenburg
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Central Michigan University, Mount Pleasant , MI
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| View Abstract PDF |
| Summary |
Calcium deficient hydroxylapatite is the mineral constituent of vertebrates’ skeletal and dental tissues. It has a similar composition and structure to apatite minerals with general formula
Ca10(PO4)6(OH,Br,F,Cl)2. The aim of our work was to elucidate the crystallization of hydroxylapatite under physiological conditions (pH7.4, 20-37°C), by Raman micro-spectroscopy. Ab initio computed Raman frequencies of fluorapatite and hydroxylapatite were also compared to the experimental results. Our study provided a further insight into the crystallization of hydroxylapatite. The combination of experimental and theoretical results provided a valuable approach to better understand how the ions’ environment affects their Raman frequencies.
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Code:
ThP02
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Time Slot/Poster Number:
008
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Session:
Biological Cells and Tissues
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Characterization by Raman spectroscopy of gold surface functionalization and immuno-specific protein binding for biosensor applications
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| Kengne Momo Rosine Pelagie1; Jeyachandran Yekkoni Lakshmana1; DANIEL Philippe1; Assaf Ali2; Esnault Charles3; Pilard Jean François3; Durand Marie josée2; Lagarde Fabienne1; Dongo Etienne4; Thouand Gérald2
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1LPEC, Le Mans, France; 2CBAC, La Roche yon, France; 3UCO2M, Le Mans, France; 4CO, Yaoundé, cameroun
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| View Abstract PDF |
| Summary |
We have developed a simple and fast electrochemical process for surface functionalization of gold and observed efficient binding of an immune-specific system of proteins. We used a combination of electrochemical quartz crystal microbalance (QCM) and Raman spectroscopy techniques to Characterize the complete system starting from surface functionalization to functional structure analysis of immobilized proteins. The surface functional groups formed a strong interaction with SpA without disturbing its functional properties. The adopted surface functionalization and analytical methodologies is anticipated to be applicable for immobilization and characterization of a wide range of biomolecules sensor applications.
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Code:
ThP02
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Time Slot/Poster Number:
009
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Session:
Biological Cells and Tissues
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Confocal Raman Microspectroscopic Studies on Living Hydrated Bacterial Biofilms
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| Truis Smith-Palmer; Christophe Sandt; David Pink
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St Francis Xavier University, Antigonish, Canada
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| View Abstract PDF |
| Summary |
Biofilms have been grown in flowcells in a Confocal Raman microspectrometer. The speed at which nutrient reached the biofilm was assessed by replacing the nutrient flow with D2O. The bright field images showed the formation of biofilm colonies. Spectra were collected from various biofilm colonies and at various depths.
Signals from nucleic acids, proteins, carbohydrates and phosholipids were distinguished. Cell rich areas and areas high in EPS were distinguished.
Signals from phospholipids were very pronounced in biofilms grown in a medium containing minimal nutrient (MN), but much less prominent when the biofilms were grown in artificial seawater (ASW).
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Code:
ThP02
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Time Slot/Poster Number:
010
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Session:
Biological Cells and Tissues
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In vivo Detection of Ferrous Cytochrome C in Mitochondria of Single Living Yeast Cells by Resonance Raman Microspectroscopy
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| Chikao Onogi; Hiro-o Hamaguchi
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Department of chemistry, School of science, The un, Tokyo, Japan
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| View Abstract PDF |
| Summary |
Resonance Raman spectra of mitochondria in living cells have been obtained with 532 nm excitation and compared to that with 632.8 nm excitation. The 532 excited Raman spectra shows sharp Raman bands at 1584, 1315, 1129,749 and 601 cm-1, in addition to known phospholipid bands. The position of these bands agree excellently with the reported resonance Raman bands of ferrous cytochrome c, which has a Q-band absorption at 520 nm. In vivo resonance Raman detection of ferrous cytochrome c provide us with real-time and quantitative tracing of respiration dynamics in mitochondria of a single living cells.
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Code:
ThP02
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Time Slot/Poster Number:
011
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Session:
Biological Cells and Tissues
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Raman Characterizing Disulfide Bonds and Secondary Structure of Bovine Serum Albumin
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| Wenlung Chen; Chich-Hsien Wang
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Department of Applied Chemistry, National Chiayi U, Chiayi, Taiwan
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| View Abstract PDF |
| Summary |
Bovine Serum Albumin is a globular protein of molecular mass ~66 kDa. It consists of 580 amino acids residues with 17 intrachain disulfide bonds . The secondary structure of BSA was reported that approximately 54% is in alpha-helix and 40% in beta-form. It is susceptible to physically and chemically denaturing treatments. How the conformational change correlates to high content of disulfide bonds in BSA draws much interest. In this report, FT-Raman was employed to elucidate the effect of disulfide bonds on the secondary structure of BSA and in turn to clarify the role of disulfide bonds on the stability of protein.
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Code:
ThP02
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Time Slot/Poster Number:
012
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Session:
Biological Cells and Tissues
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Probing tumour and peritumoral tissues in superficial and nodular skin basal cell carcinoma using polarized Raman microspectroscopy
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| Olivier Piot; Elodie Ly; Michel Manfait
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UMR 6237 MEDyC, Université de Reims Champagne Arde, Reims, France
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| View Abstract PDF |
| Summary |
Polarized Raman microspectroscopy can provide precious information regarding the orientation and ordering of the molecules in a sample without staining or particular preparation. This study reports the use of polarized Raman microspectroscopy on the nodular and superficial types of basal cell carcinoima to discriminate between healthy epidermis and tumour, and between normal and peritumoral stroma. Depolarization ratios and hierarchical cluster analysis demonstrate that polarized Raman microspectroscopy can better identify the tumour and the peritumoral dermis than conventional Raman microspectroscopy, and hence gives potential complementary data about their molecular characteristics (molecular composition, secondary structure of proteins, intra- and/or inter-molecular bonding).
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Code:
ThP02
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Time Slot/Poster Number:
013
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Session:
Biological Cells and Tissues
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Micro-Raman Detection of Nuclear Membrane Lipid Fluctuations in Senescent Cancer Cells
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| Melissa Mariani1; Lindsey Maccoux2, 4; Christian Matthäus3; Max Diem5; Jan Hengstler4; Volker Deckert3, 6
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1Mount Sinai School of Medicine, New York, NY; 2ISAS-Institute for Analytical Sciences, Dortmund, Germany; 3IPHT-Institute of Photonic Technology, Jena, Germany; 4Leibniz Research Centre (IfADo), Dortmund, Germany; 5Northeastern University, Boston, MA; 6FSU- Jena, Jena, Germany
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| View Abstract PDF |
| Summary |
Originally identified in cultured cells, oncogenic cellular senescence is a growth-arrest mechanism which may inhibit tumor development. A further understanding of senescence will provide valuable implications for improving treatment targets. Yet, many associated mechanisms between proliferating cells and senescent cells remain to be thoroughly understood.Through the use of label-free micro-Raman spectroscopy, control and senescent cells were non-invasively imaged. Resulting spectral images were processed using chemometric techniques and average nuclei spectra from each sample set were compared. In turn, changes in the –cis and –trans unsaturated lipid isomer content were found to differ among proliferating and senescent cells.
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Code:
ThP02
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Time Slot/Poster Number:
014
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Session:
Biological Cells and Tissues
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Chemometric Analysis of Raman Spectra of Lactobacilli Isolated from Kefir
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| Cuauhtemoc Araujo Andrade1; Pablo Mobili2; Claudio Frausto Reyes3; Esteban Gerbino2; Graciela De Antoni2; Rumen Ivanov Tzonchev1; Andrea Gomez Zavaglia2
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1UNIDAD ACADEMICA DE FISICA DE LA UNIVERSIDAD AUTON, Zacatecas, Mexico; 2CIDCA, Conicet La Plata, UNLP, La Plata, Argentina; 3CIO, unidad Aguascalientes, Aguascalientes, Mexico
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| View Abstract PDF |
| Summary |
The aim of the present work was to develop an approach based on Raman spectroscopy in combination with multivariate analysis for a rapid differentiation of L. kefir from other phytogenetically related lactobacilli present in kefir grains.
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Code:
ThP02
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Time Slot/Poster Number:
015
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Session:
Biological Cells and Tissues
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Interaction Of Kyotorphin In Different Concentrations With The Membrane Of Optically Trapped DMPC Vesicle
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| Gönül Basar1; Günay Basar2; Seda Kın2; Ugur Parlatan1; Seyma Seninak1
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1Istanbul University, Istanbul, Turkey; 2Istanbul Technical University, Istanbul, Turkey
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| View Abstract PDF |
| Summary |
Interaction of Kyotorphin with the membrane of optically trapped DMPC vesicle was investigated by using a Raman Tweezers Spectrometer. The interaction of DMPC vesicles with different KTP concentrations and whether the concentration of KTP increases the degree of the membrane disorder were investigated. Peak intensity ratio of 1086/1065 cm-1, the gauche-to-trans, was used to monitor changes in membrane order.
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Code:
ThP02
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Time Slot/Poster Number:
016
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Session:
Biological Cells and Tissues
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Ionizing Radiation Induces Cell Death Observed By FTIR And Raman Micro-spectral Imaging
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| Qing Huang1; Zhigang Ke1; Zeming Qi2; Guangfu Chen3; Zeliang Yu1
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1Chinese Academy of Sciences, Hefei, China; 2University of Science and Technology of China, Hefei, China; 3Anhui Medical University, Hefei, China
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| View Abstract PDF |
| Summary |
Ionizing radiation exists ubiquitously in nature and affects life profoundly. Our lab has been investigating ionizing radiation induced biological effects for decades and in this study we investigated ionizing radiation induced death of HeLa cells through FTIR and Raman confocal microscopy. Through micro-spectral imaging, both direct and indirect interactions involving ROS (reactive oxygen species) free radicals attack on organelles in the cells were observed and discussed.
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Code:
ThP02
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Time Slot/Poster Number:
018
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Session:
Biological Cells and Tissues
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Effects of Normalization on Spectral Unmixing and Clustering Algorithms in Raman Imaging
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| Martin Hedegaard1; Christian Matthäus2; Søren Hassing1; Christoph Krafft2; Max Diem3; Jürgen Popp2, 4
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1University of Southern Denmark, SENSE, Odense M, Denmark; 2Institute of Photonic Technology, Jena, Germany; 3Northeastern University, Boston, United States; 4Institute of Physical Chemistry, University Jena, Jena, Germany
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| View Abstract PDF |
| Summary |
Multivariate image analysis of Raman datasets are frequently used to extract chemical information from Raman datasets. We discuss the effects of normalization of the data with both clustering and spectral unmixing algorithms.
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Code:
ThP02
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Time Slot/Poster Number:
020
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Session:
Biological Cells and Tissues
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Surface-enhanced Raman scattering (SERS) spectra of hemoglobin of mouse and rabbit with self-assembled nano silver film
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| Yipu Kang; Minzhen Si; Renming Liu
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Chuxiong Normal University, Chuxiong, China
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| View Abstract PDF |
| Summary |
Nano silver film was prepared by electrolysis method. The transmission electron microscopy and scanning electron microscopy were employed to detect the morphology of the silver particles. The SERS spectra of the hemoglobin (rabbit and mouse) on nano silver film were gained. It known from the SERS spectra that the nano silver films could enhance the Raman signal of the hemoglobin efficiently, and the sodium citrate and PBS create no influence to the SERS spectra of the hemoglobin. The electrolysis technique to fabricate this highly bio-active, stable, reusable, and low-cost SERS substrate will be useful in the development of hemoglobin detection.
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Code:
ThP02
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Time Slot/Poster Number:
021
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Session:
Biological Cells and Tissues
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Identification of Arbuscular Mycorrhizal Fungal (AMF) Spore Components
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| Katarzyna M. Marzec1; Marta Murowana1; Katarzyna Turnau2; Leonard Proniewicz1; Malgorzata Baranska1
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1Faculty of Chemistry, Jagiellonian University, Krakow, Poland; 2Faculty of Biology and Earth Sciences, UJ, Krakow, Poland
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| View Abstract PDF |
| Summary |
Genus Glomus includes arbuscular mycorrhizal fungi (AMF). Identification of the AMF spores has traditionally been carried out on the basis of their morphology. However, this method as well as molecular identification does not always give satisfactory results. In this work, Raman spectroscopy was applied to analyse fungal spores of selected representatives of the Glomus-A group (G. caledonium, G. corantum, G. geosporum and G. verruculosum). The main goal of this study was a quantitative analysis of spores component, but the attempt to identify and recognize the four Glomus species by cluster analysis have also been made.
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