Abstract Session Viewer

ThP08

Code: ThP08	Time Slot/Poster Number: 099	Session: SERS applications II
Analysis of DNA-polyamine interactions using SERS-coated microplates in a dedicated Raman plate reader.
Igor Zlatkin¹; David Drapcho¹; Stuart Farquharson²
¹Digilab, Inc., Holliston, MA; ²Real-Time Analyzers, Middletown, CT

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Summary
We investigated SERS and Raman spectra of several natural polyamines alone, and in combination with short (smaller than 200 bp) DNA fragments at different pH, DNA/polyamine ratio and in the presence of a variety of tertiary components, for evidence of conformational changes induced by interactions of the polyamines with the short DNA fragments. Commercially available SERS-active microtiter plates (Real-Time Analyzers, Middletown, CT) with a dedicated Raman Plate Reader ("Identity" from Digilab, Holliston, MA), the combination of which enables the routine high throughput SERS analysis of compounds at low concentrations, were used.

Code: ThP08	Time Slot/Poster Number: 101	Session: SERS applications II
Surface-enhanced Raman spectroscopy: Substrates and Analyzers You Can Use
Frank Inscore; Chetan Shende; Atanu Sengupta; Hermes Huang; Stuart Farquharson
Real-Time Analyzers, Inc., Middletown, CT

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Summary
Since the mid-1990’s there has been a resurgence in SERS-based research with the detection of single-molecules and the acknowledgement of “hot-spots”. These measurements provoked new examination of SERS theory with a focus on the structure of these hot spots: fractal clusters, edges, or inter-particle gaps. Meanwhile, Real-Time Analyzers has been developing SERS-active sample systems and analyzers to exploit this phenomenon for trace chemical analysis. This presentation reviews the analytical capabilities and limitations for SERS-active substrates and their application to analysis of poisons in water supplies, food contamination, drug and explosives detection and proteomics.

Code: ThP08	Time Slot/Poster Number: 102	Session: SERS applications II
Surface-Enhanced Raman Spectroscopy Of Isoflavones With Silver-Doped Nano-Porous Inorganic Substrates
Ryo Sekine¹; Naomi Lewcenko²; Leone Spiccia²; Evan Robertson³; Don McNaughton¹
¹Centre for Biospectroscopy, Monash University, Clayton, Australia; ²School of Chemistry, Monash University, Clayton, Australia; ³School of Molecular Sciences, La Trobe University, Bundoora, Australia

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Summary
Surface-enhanced Raman spectroscopy (SERS) of several common isoflavones are presented, acquired using citrate-reduced silver colloids. The spectra show highly characteristic profiles depending on the OH substitution pattern, as these groups are key participants in the adsorption process. We extend this work to using alternative substrates, in particular, silver-doped porous titania, to broaden its scope towards future applications.

Code: ThP08	Time Slot/Poster Number: 103	Session: SERS applications II
SERS optimization of gold nanocylinder arrays: influence of the surrounding medium and application for Polycyclic Aromatic Hydrocarbons detection
Nicolas Guillot¹; Hong Shen²; Salem Ben Amor¹; Catalina David¹; Olivier Péron³; Emmanuel Rinnert³; Timothée Toury²; Marc Lamy de la Chapelle¹
¹Université Paris 13, Bobigny, France; ²Université de Technologie de Troyes, Troyes, France; ³IFREMER, Plouzané, France

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Summary
This study describes the effect of the surrounding medium on the SERS efficiency using nanolithographied substrates on which Polycyclic Aromatic Hydrocarbons are investigated. We will show that the optimum nanocylinder size is shifted to lower diameter by increasing the dielectric constant of the liquid medium. This rule is discussed in terms of Localized Surface Plasmon Resonance since its position influences directly SERS intensity. This study is done for two excitation wavelengths: 632.8 nm and 785 nm. The aim of this work is collect information in order to product future active SERS sensors suitable for in situ environmental analysis.

Code: ThP08	Time Slot/Poster Number: 104	Session: SERS applications II
Advanced Gas Sensors Using SERS-Activated Waveguides
Robert Lascola; Scott McWhorter; Simona Hunyadi Murph
Savannah River National Laboratory, Aiken, SC

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Summary
This contribution describes progress towards the development and testing of a functionalized capillary that will provide detection of low-concentration gas-phase analytes through SERS. Measurement inside a waveguide allows interrogation of a large surface area, potentially overcoming the short distance dependence of the SERS effect.

Code: ThP08	Time Slot/Poster Number: 105	Session: SERS applications II
On The Molecular Origin Of Bacterial SERS Spectra
W. Ranjith Premasiri; Larry Ziegler
Boston University, Boston, MA

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Summary
The molecular origin of the SERS spectra of bacteria has been established. Due to the nature of the SERS enhancement mechanisms the SERS bacterial signals must be derived from molecular components at the outer layer of the cell walls. Establishing the chemical identity of these species/strain specific SERS signals can maximize the effectiveness of this approach by improving the reproducibility and robustness of this technique. Experimental observations suggest that the SERS spectra of many bacteria originate from a chemical compound(s) containing adenine or adenine-like moiety. These accounts for the prominent ~737 cm-1 band observed in most bacterial SERS spectra.

Code: ThP08	Time Slot/Poster Number: 106	Session: SERS applications II
Surface-Enhanced Raman Scattering of Proteins
Mehmet Kahraman; Iknur Sur; Mustafa Culha
Yeditepe University, Istanbul, Turkey

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Summary
Convective assembly method is used to assemble of proteins and AgNPs to obtain reproducible SERS spectra for protein detection and identification. The assembled protein-AgNP structure is characterized with AFM and SEM, and it is found that the formation of the assemblies strongly depends on charge and concentration of the proteins. The CV% for ten spectra is calculated and found as in the range of 15-25. The results indicate that, it is possible to detect proteins down to 0.5 µg/mL. These results demonstrate that, this technique can be used for the sensitive and reproducible protein detection and identification using SERS.

Code: ThP08	Time Slot/Poster Number: 107	Session: SERS applications II
Band Shifts in Surface Enhanced Raman Spectra of Indolic Molecules Adsorbed on Gold Colloids
Qiang Tu¹; Jonathan Eisen²; Chang Chang^{1, 3}
¹Drexel University, Philadelphia, Pennsylvania; ²Washington University, St. Louis, Missouri; ³University of Pennsylvania, Philadelphia, Pennsylvania

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Summary
Serotonin is an important neurotransmitter and immunomodulator. Localizing serotonin and its related molecules is imperative in biomedical research. Here we demonstrated that surface enhanced Raman spectroscopy (SERS) is capable of detecting indolic molecules including serotonin, tryptophan, melatonin and 5-HIAA. Their indole related vibrational modes and Raman band shifts are analyzed.

Code: ThP08	Time Slot/Poster Number: 108	Session: SERS applications II
Raman and Surface-Enhanced Raman for Military Applications
Jason Guicheteau^{1, 2}; Steven Christesen^{1, 2}; Ashish Tripathi^{1, 4}; Erik Emmons^{1, 3}; Darren Emge^{1, 2}; Phillip Wilcox^{1, 2}; Augustus, W. Fountain III^{1, 2}
¹, Apg, MD; ²USA RDECOM ECBC, APG, MD; ³NRC Research Associateship, ECBC, APG, MD; ⁴SAIC, APG, MD

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Summary
The Laser Standoff Detection Branch at the Edgewood Chemical Biological Center (ECBC) has an active research program on the applications of Raman and Surface-enhanced Raman to the detection of chemical, biological, and explosive materials. A broad overview of current research will be presented including the applications of spatially offset Raman spectroscopy, Raman and surface-enhanced Raman chemical imaging of explosives and identification of bacteria, the development of SERS molecular recognition elements and applications of Raman spectroscopy for standoff detection of agent materials.

Code: ThP08	Time Slot/Poster Number: 109	Session: SERS applications II
Surface-enhanced Raman Spectroscopy for Studying the Tensile Structure Between Au@Pd Nanoparticle Interfaces
Ping-Ping Fang; Jian-Feng Li; Xiao-Dong Lin; Sai Duan; Bin Ren; De-Yin Wu; Zhong-Qun Tian
State Key Lab. of Phys. Chem. of Solid Surfaces, Xiamen, China

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Summary
SERS was used to detect the electronic structure of the Au-Pd interface. Two probe molecules were used to investigate the potential dependent interfacial information. it was found that when all the other conditions were the same, the Raman shift of the probe molecule varied with the change of the Pd shell thickness. However, at a same Pd shell thickness, the Raman shift of the C-S stretching did not change obviously with the size of the Au core.

Code: ThP08	Time Slot/Poster Number: 110	Session: SERS applications II
Nanoparticle Encapsulated Hydrogel for SERS Measurement
Ka Yeong Shin; Kyungtag Ryu; Hoik Lee; Jinwoo Kim; Daewon Sohn; Hoeil Chung
Hanyang University, Seoul, South Korea

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Summary
The major goal of this research is to develop a novel SERS substrate that can be used for reliable quantitative analysis. Initially we prepared silica coated gold nanoparticles (Au@SiO2) and then these particles were encapsulated into a hydrogel via polymerization with acrylic acid under irradiation of γ-rays. After nanoparticles encapsulated hydrogel was performed, the silica layer surrounding gold nanoparticle was partially etched by hydrofluoric acid to enable a target analyte (2-naphthalenethiol) to access onto gold surface. Since hydrogel is easy to swell at neutral and basic condition, it is an excellent polymeric molecular trap for SERS measurement.

Code: ThP08	Time Slot/Poster Number: 111	Session: SERS applications II
Surface-Enhanced Raman Scattering (SERS) Detection of a Bioactive Mediator
Kyle Bantz; Audrey Guerard; Christy Haynes
University of Minnesota, Minneapolis, MN

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Summary
Surface-enhanced Raman spectroscopy is a powerful signal transduction mechanism since detection can be accomplished in aqueous solutions without interfering signal from water, and unique molecular spectra can be obtained from structurally similar analytes. This makes SERS an ideal analytical technique for the detection and discrimination between similar phospholipids and secreted bioactive mediators such as platelet-activating factor. To facilitate SERS detection of phospholipids, an alkanethiol monolayer is assembled onto the substrates to act as a partition layer, which concentrates the lipids within the zone of enhancement. The monolayer allows detection of biologically relevant concentrations and distinction of specific lipids within mixtures.

Code: ThP08	Time Slot/Poster Number: 112	Session: SERS applications II
SERS-based Mechanical Nano-Sensor for Mutiplexed Protein Detection
K.W. Kho¹; C.Y. Fu²; U.S. Dinish²; T. Praveen²; M.C. Olivo^{2, 3}
¹National Cancer Centre, Singapore, Singapore; ²Singapore Bioimaging Consortium, Singapore, Singapore; ³National University Ireland, Galway, Ireland

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Summary
A problem in conventional SERS-based multiplexed immunoassay is the requirement of multiple washing steps and the use of secondary antibodies, hence pro-longing overall analysis time, and increasing material wastage. Here, we present a novel SERS-readout concept in which the SERS-reporter molecule is covalently sandwiched in-between the primary capturing antibody and the SERS substrate. Detection of antigen-antibody binding event is achieved by monitoring changes in the reporter’s SERS spectrum occurred as the molecule experienced binding-induced stress exerted by the antigen-antibody complex - thus, the reporter molecule is now acting as a nano-scale mechanical sensor. We will discuss such an interesting concept.

Code: ThP08	Time Slot/Poster Number: 113	Session: SERS applications II
New SERS Substrates For Polycyclic Aromatic Hydrocarbon (PAH) Detection: Towards Quantitative SERS Sensors For Environmental Analysis.
Olivier Péron¹; Emmanuel Rinnert¹; Chantal Compère¹; Timothée Toury²; Marc Lamy de la Chapelle³
¹Service Interfaces et Capteurs, RDT, Ifremer, Plouzané, France; ²LNIO-Institut Charles Delaunay, FRE 2848, UTT, Troyes, France; ³CSPBAT (FRE 3043)-Université Paris XIII, Bobigny, France

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Summary
In the investigation of chemical pollutions, such as PAHs (Polycyclic Aromatic Hydrocarbons) at low concentration in aqueous medium, surface-enhanced Raman scattering (SERS) stands for an alternative to the inherent low cross-section of normal Raman scattering. Indeed, SERS is a very sensitive spectroscopic technique due to the excitation of the surface plasmon modes of the nanostructured metallic film. Hydrophobic SERS substrates based on polystyrene beads surrounded by gold nanoparticles could be used as quantitative SERS sensors for environmental analysis.

Code: ThP08	Time Slot/Poster Number: 114	Session: SERS applications II
Targeted SERS Nanosensors for Time-Lapse Microscopy of Live Cell Response to Drug-Based Stimuli
Kristy Nowak-Lovato; Kirk Rector
Los Alamos National Laboratory, Los Alamos, NM

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Summary
Gold-core, silver-shell surface-enhanced Raman Scattering (SERS) nanosensor that has both a targeting component as well as a sensing component is reported. Ligand 4-mercaptopyridine (4-MPy) on the particle surface enables pH sensitivity. 2,4-ε-dinitrophenol-L-lysine (DNP) ligands on the nanoparticle surface enables targeting of the nanosensor through the FcεRI receptor-mediated endocytic pathway. Use of these techniques allows for dynamic changes in the ensemble pH distributions inside a single complete cell to be recorded. In addition, with this capability, the ability to record the pH changes in response to drug based stimuli (amiloride or bafilomycin) when H+ flux across endosomal membranes is effected.

Code: ThP08	Time Slot/Poster Number: 115	Session: SERS applications II
Ascertaining p,p′-dimercaptoazobenzene produced from p-aminothiophenol by selective catalytic coupling reaction on silver nanoparticles
Mengtao Sun
Institute of Physics, Chinese Academy of sciences, Beijing, China

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Summary
Combining experiment and theory, evidence from surface enhanced Raman scattering (SERS) were obtained for p,p′-dimercaptoazobenzene (DMAB) produced from p-aminothiophenol (PATP) by selective catalytic coupling reaction on silver nanoparticles. The time-dependent SERS spectra of PATP are consistent with the calculated SERS spectra of DMAB, which is the direct evidence for the production of DMAB from PATP by selective catalytic coupling reaction on silver nanoparticles. The so called “b2 modes” of PATP is the -N=N- related vibrational modes of DMAB. By analyzing the symmetry of strong enhanced vibrational modes, it is derived that six strong vibrational modes are enhanced by Plasmon.

Code: ThP08	Time Slot/Poster Number: 116	Session: SERS applications II
Sensitive detection of melamine using surface enhanced Raman spectroscopy on solid substrates
Evelyn Kämmer³; Anne März³; Paulo Augusto Da Costa Filho²; Fabien Robert²; Jürgen Popp^{1, 3}; Petra Rösch³
¹Institute of Photonic Technology (IPHT), Jena, Germany; ²Nestlé Research Center Lausanne, Lausanne, Switzerland; ³Friedrich-Schiller University, Jena, Germany

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Summary
Melamine is a nitrogen rich substance and it is used as additive in food products, especially in milk products, to simulate higher protein content. Moreover melamine is a toxic substance and causes tissue damage through the formation of crystals in the kidneys. Therefore, it is extremely important to detect it quickly and at low concentrations. Surface enhanced Raman spectroscopy is particularly well suited for the detection of melamine. Here enzymatically reduced silver substrates are used for rapid detection of Melamine at low concentrations.

Code: ThP08	Time Slot/Poster Number: 117	Session: SERS applications II
ID-SERS Based Reference Method for Quantification of Large Biomolecules on a Single Chip
Fatemeh Yaghobian; Rainer Stosch
Physikalisch-Technische Bundesanstalt (PTB), Braunschweig, Germany

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Summary
Accuracy and precision of quantitative SERS results have been significantly increased by applying a method based on the isotope-dilution (ID) principle. In this ID-SERS approach, an isotopically labelled target molecule is spiked to the sample at a known concentration.The ID-SERS approach has been realized as a “one-chip” approach using “Bio-chips” made of intrinsically grown spherical silver nanoparticles with gaps less than 10 nm in between. In addition, the scope of the method has been extended to larger biomolecules like peptides which will be shown using the example of the human growth-hormone peptide T12 at physiologically relevant serum concentrations.

Code: ThP08	Time Slot/Poster Number: 118	Session: SERS applications II
Trace Level Detection of Water Contamination by SERS
Sasani Jayawardhana; Alex Mazzolini; Paul Stoddart
Swinburne University of Technology, Hawthorn, Australia

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Summary
The detection of atrazine in an aqueous solution has been demonstrated down to a concentration of 0.1 µg/ml (100 ppb) based on a simple spot test using the technique of surface-enhanced Raman scattering. Work is underway to further optimize the substrate which is a silver nanorod structure fabricated using oblique angle deposition. This holds promise to improve adsorption of the target molecule on the substrate and reduce the detection limit even further, thereby enabling potential field testing applications.

Code: ThP08	Time Slot/Poster Number: 119	Session: SERS applications II
Cancer diagnosis application with fluorescence-SERS dual modal nanoprobes
Sangyeop Lee; Hyangah Chon; Juhui Ko; Jaebum Choo
Dept. of Bionano Engineerng, Hanyang University, Ansan Si, South Korea

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Summary
The application of metal nanoprobes is emerging as one of the most effective optical imaging tools in the biomedical cancer diagnosis. For example, quantum dots are extensively used as effective fluorescent labels for cellular imaging. However, most of currently available fluorescent organic dyes have relatively weak emission intensities and they are also photo-bleached quickly. In order to resolve this problem, more sensitive and stable probes are still needed. In the present work, dual modal nanopronbes, which can be used for both the Surface-enhanced Raman scattering (SERS) and fluorescence detections, have been developed.

Code: ThP08	Time Slot/Poster Number: 120	Session: SERS applications II
On the way to reusable SERS-Arrays in (bio)analytic
Karina Weber¹; Dana Cialla¹; Uwe Huebner²; Matthias Zeisberger²; Henrik Schneidewind²; Konstantin Kirsch²; Roland Mattheis²; Robert Moeller¹; Juergen Popp^{1, 2}
¹Friedrich-Schiller-University Jena, Jena, Germany; ²Institute of Photonic Technology, Jena, Germany

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Summary
Regular 3D patterned metallized nanostructures are of high interest in advanced optical sensing and other applications. The strong field enhancement due to the excitation of localized and propagating surface plasmon polaritons is used for the effective enhancement of the Raman signals (surface enhanced Raman spectroscopy – SERS). Within this contribution a fabrication technique using a silver metal deposition on a prepatterned substrate is presented. This allows different preparation and material parameters as well as reuse of the substrates. Thus, the EBL process can be applied more cost-efficient production strategies for fabrication of SERS substrates will be investigated.